DENND4B Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14273R-Cy5.5 |
| Product Name | DENND4B Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DENND4B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9909 |
| Swiss Prot | O75064 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DEN4B_HUMAN; DENN domain-containing protein 4B; DENN/MADD domain containing 4B; Dennd4b; hCG_18235; KIAA0476. |
| Background | Guanine nucleotide exchange factor (GEF) which may activate RAB10. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DENND4B Polyclonal Antibody, Cy5.5 Conjugated (BS-14273R-CY5-5) validated for, and at what dilution?
BS-14273R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DENND4B Polyclonal Antibody, Cy5.5 Conjugated (BS-14273R-CY5-5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig.
How should BS-14273R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DENND4B Polyclonal Antibody, Cy5.5 Conjugated (BS-14273R-CY5-5)?
KLH conjugated synthetic peptide derived from human DENND4B
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-14273R-CY5-5)?
Cy5.5 on BS-14273R-CY5-5: excitation / emission 675nm/694nm.