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DFNA5 Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
Overview
Catalog # bs-14286R-PerCP-100uL
Product Name DFNA5 Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DFNA5
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1687
Swiss Prot O60443
Subcellular location Cytoplasm
Synonyms ICERE-1; Non-syndromic hearing impairment protein 5; Inversely correlated with estrogen receptor expression 1; DFNA5; ICERE1
Background Involved in apoptosis and cell survival. Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is DFNA5 Polyclonal Antibody, PerCP Conjugated (bs-14286R-PerCP) validated for, and at what dilution?

bs-14286R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DFNA5 Polyclonal Antibody, PerCP Conjugated (bs-14286R-PerCP) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep.

How should bs-14286R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DFNA5 Polyclonal Antibody, PerCP Conjugated (bs-14286R-PerCP)?

KLH conjugated synthetic peptide derived from human DFNA5

What are the excitation and emission wavelengths of the PerCP conjugate (bs-14286R-PerCP)?

PerCP on bs-14286R-PerCP: excitation / emission 482nm/677nm.