DGKI Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-14299R-BF350 |
| Product Name | DGKI Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DGKI |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9162 |
| Swiss Prot | O75912 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DAG kinase iota; DGK iota; DGKiota; DGKI_HUMAN; Diacylglycerol kinase iota;; Diglyceride kinase iota. |
| Background | This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DGKI Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-14299R-BF350) validated for, and at what dilution?
bs-14299R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DGKI Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-14299R-BF350) react with?
Tested reactivity: Human.
How should bs-14299R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DGKI Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-14299R-BF350)?
KLH conjugated synthetic peptide derived from human DGKI
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-14299R-BF350)?
AbBy Fluor-350 on bs-14299R-BF350: excitation / emission 346nm/442nm.