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DGKI Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
Overview
Catalog # bs-14299R-HRP-100uL
Product Name DGKI Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Human
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DGKI
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9162
Swiss Prot O75912
Subcellular location Cytoplasm, Nucleus
Synonyms DAG kinase iota; DGK iota; DGKiota; DGKI_HUMAN; Diacylglycerol kinase iota;; Diglyceride kinase iota.
Background This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is DGKI Polyclonal Antibody, HRP Conjugated (bs-14299R-HRP) validated for, and at what dilution?

bs-14299R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DGKI Polyclonal Antibody, HRP Conjugated (bs-14299R-HRP) react with?

Tested reactivity: Human.

How should bs-14299R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DGKI Polyclonal Antibody, HRP Conjugated (bs-14299R-HRP)?

KLH conjugated synthetic peptide derived from human DGKI