GNAI3 Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-1429R-100uL |
| Product Name | GNAI3 Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GNAI3 |
| Immunogen Range | 241-354/354 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 2773 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | GAI3; 87U6; FLJ26559; G protein alpha inhibiting 3; Gi alpha 3; Gi alpha-3; GNAI3; GNAI3_HUMAN; Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 3; Guanine nucleotide binding protein Gk alpha subunit; Guanine nucleotide-binding protein Gk subunit alpha; OTTHUMP00000013368; Gnai3; Guanine nucleotide binding protein G protein, alpha inhibiting 3; G Alpha i-3. |
| Background | GAI3 is the a subunit of inhibitory trimeric G protein (Gi), which inhibits adenylate cyclase once its G protein coupled receptors (GPCR) such as a2 adrenergic receptors are activated. GAI3 can be inactivated by pertussis toxin. Guanine nucleotide binding proteins are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor regulated K(+) channels. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GNAI3 Polyclonal Antibody (bs-1429R) validated for, and at what dilution?
bs-1429R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-1429R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GNAI3 Polyclonal Antibody (bs-1429R)?
KLH conjugated synthetic peptide derived from human GNAI3 Immunogen range: 241-354/354.