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GNAI3 Polyclonal Antibody, Biotin Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-1429R-Biotin-100uL
Product Name GNAI3 Polyclonal Antibody, Biotin Conjugated
Applications
ELISA IHC-P IHC-F
Predicted Reactivity Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GNAI3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 2773
Subcellular location Cytoplasm, Cell membrane
Synonyms GAI3; 87U6; FLJ26559; G protein alpha inhibiting 3; Gi alpha 3; Gi alpha-3; GNAI3; GNAI3_HUMAN; Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 3; Guanine nucleotide binding protein Gk alpha subunit; Guanine nucleotide-binding protein Gk subunit alpha; OTTHUMP00000013368; Gnai3; Guanine nucleotide binding protein G protein, alpha inhibiting 3; G Alpha i-3.
Background GAI3 is the a subunit of inhibitory trimeric G protein (Gi), which inhibits adenylate cyclase once its G protein coupled receptors (GPCR) such as a2 adrenergic receptors are activated. GAI3 can be inactivated by pertussis toxin. Guanine nucleotide binding proteins are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor regulated K(+) channels.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is GNAI3 Polyclonal Antibody, Biotin Conjugated (bs-1429R-Biotin) validated for, and at what dilution?

bs-1429R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-1429R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GNAI3 Polyclonal Antibody, Biotin Conjugated (bs-1429R-Biotin)?

KLH conjugated synthetic peptide derived from human GNAI3

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