DGKK Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14300R-BF488 |
| Product Name | DGKK Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DGKK |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 139189 |
| Swiss Prot | Q5KSL6 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | 142 kDa diacylglycerol kinase; DAG kinase kappa; DGK kappa; DGKK_HUMAN; Diacylglycerol kinase kappa; Diglyceride kinase kappa. |
| Background | The protein encoded by this gene is an enzyme that phosphorylates diacylglycerol, converting it to phosphatidic acid. The encoded protein is a membrane protein and is inhibited by hydrogen peroxide. Variations in this gene have been associated with hypospadias. [provided by RefSeq, Mar 2011] |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DGKK Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14300R-BF488) validated for, and at what dilution?
bs-14300R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DGKK Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14300R-BF488) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse.
How should bs-14300R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DGKK Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14300R-BF488)?
KLH conjugated synthetic peptide derived from human DGKK
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-14300R-BF488)?
AbBy Fluor-488 on bs-14300R-BF488: excitation / emission 499nm/519nm.