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DCTN5 Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-14308R-APC
Product Name DCTN5 Polyclonal Antibody, APC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Pig, Horse
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DCTN5
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 84516
Swiss Prot Q9BTE1
Subcellular location Cytoplasm
Synonyms Dynactin subunit 5; Dynactin subunit p25; DCTN5
Background This gene encodes a subunit of dynactin, a component of the cytoplasmic dynein motor machinery involved in minus-end-directed transport. The encoded protein is a component of the pointed-end subcomplex and is thought to bind membranous cargo. A pseudogene of this gene is located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is DCTN5 Polyclonal Antibody, APC Conjugated (bs-14308R-APC) validated for, and at what dilution?

bs-14308R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DCTN5 Polyclonal Antibody, APC Conjugated (bs-14308R-APC) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Pig, Horse.

How should bs-14308R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DCTN5 Polyclonal Antibody, APC Conjugated (bs-14308R-APC)?

KLH conjugated synthetic peptide derived from human DCTN5

What are the excitation and emission wavelengths of the APC conjugate (bs-14308R-APC)?

APC on bs-14308R-APC: excitation / emission 650nm/660nm.