DHX36 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14319R-BF488 |
| Product Name | DHX36 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DHX36 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 170506 |
| Swiss Prot | Q9H2U1 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DDX36; DEAH box protein 36; Dhx36; DHX36_HUMAN; EC 3.6.1.-; KIAA1488; MLE like protein 1; MLE-like protein 1; MLEL1; Probable ATP dependent RNA helicase DHX36; Probable ATP-dependent RNA helicase DHX36; RHAU; RNA helicase associated with AU rich element ARE; RNA helicase associated with AU-rich element ARE. |
| Background | This gene is a member of the DEAH-box family of RNA-dependent NTPases which are named after the conserved amino acid sequence Asp-Glu-Ala-His in motif II. The protein encoded by this gene has been shown to enhance the deadenylation and decay of mRNAs with 3'-UTR AU-rich elements (ARE-mRNA). The protein has also been shown to resolve into single strands the highly stable tetramolecular DNA configuration (G4) that can form spontaneously in guanine-rich regions of DNA. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DHX36 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14319R-BF488) validated for, and at what dilution?
bs-14319R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DHX36 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14319R-BF488) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Horse, Rabbit.
How should bs-14319R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DHX36 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14319R-BF488)?
KLH conjugated synthetic peptide derived from human DHX36
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-14319R-BF488)?
AbBy Fluor-488 on bs-14319R-BF488: excitation / emission 499nm/519nm.