DIAPH3 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14325R-Cy5 |
| Product Name | DIAPH3 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DIAPH3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 81624 |
| Swiss Prot | Q9NSV4 |
| Subcellular location | Cytoplasm |
| Synonyms | AN; AUNA1; Dia2; diap3; DIAP3_HUMAN; DIAPH3; Diaphanous homolog 3 (Drosophila); Diaphanous homolog 3; Diaphanous related formin 3; Diaphanous-related formin-3; DKFZp434C0931; DKFZp686A13178; DRF3; FLJ34705; mDia2; NSDAN; OTTHUMP00000018480; Protein diaphanous homolog 3; RP11-26P21.1. |
| Background | Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DIAPH3 Polyclonal Antibody, Cy5 Conjugated (bs-14325R-Cy5) validated for, and at what dilution?
bs-14325R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DIAPH3 Polyclonal Antibody, Cy5 Conjugated (bs-14325R-Cy5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Horse, Chicken.
How should bs-14325R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DIAPH3 Polyclonal Antibody, Cy5 Conjugated (bs-14325R-Cy5)?
KLH conjugated synthetic peptide derived from human DIAPH3
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-14325R-Cy5)?
Cy5 on bs-14325R-Cy5: excitation / emission 625,650nm/670nm.