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DIP2B Polyclonal Antibody, AbBy Fluor-647 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Rabbit
Overview
Catalog # bs-14332R-BF647
Product Name DIP2B Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Rabbit
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DIP2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 57609
Swiss Prot Q9P265
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms DIP2 homolog B; DIP2; DIP2B_HUMAN; DIP2 disco interacting protein 2 homolog B (Drosophila); Disco interacting protein 2 homolog B; FLJ34278; KIAA1463; MGC104005.
Background This gene encodes a member of the disco-interacting protein homolog 2 protein family. The encoded protein contains a binding site for the transcriptional regulator DNA methyltransferase 1 associated protein 1 as well as AMP-binding sites. The presence of these sites suggests that the encoded protein may participate in DNA methylation. This gene is located near a folate-sensitive fragile site, and CGG-repeat expansion in the promoter of this gene which affects transcription has been detected in individuals containing this fragile site on chromosome 12. [provided by RefSeq, Aug 2011]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is DIP2B Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-14332R-BF647) validated for, and at what dilution?

bs-14332R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DIP2B Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-14332R-BF647) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Rabbit.

How should bs-14332R-BF647 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DIP2B Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-14332R-BF647)?

KLH conjugated synthetic peptide derived from human DIP2

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-14332R-BF647)?

AbBy Fluor-647 on bs-14332R-BF647: excitation / emission 650nm/665nm.