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DNAJB12 Polyclonal Antibody, APC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-14371R-APC
Product Name DNAJB12 Polyclonal Antibody, APC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DNAJB12
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 54788
Swiss Prot Q9NXW2
Subcellular location Cell membrane
Synonyms DJ10; DnaJ homolog subfamily B member 12; DNAJB12
Background DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM, Mar 2008]
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is DNAJB12 Polyclonal Antibody, APC Conjugated (bs-14371R-APC) validated for, and at what dilution?

bs-14371R-APC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DNAJB12 Polyclonal Antibody, APC Conjugated (bs-14371R-APC) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse.

How should bs-14371R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DNAJB12 Polyclonal Antibody, APC Conjugated (bs-14371R-APC)?

KLH conjugated synthetic peptide derived from human DNAJB12

What are the excitation and emission wavelengths of the APC conjugate (bs-14371R-APC)?

APC on bs-14371R-APC: excitation / emission 650nm/660nm.