phospho-Dnmt3a (Ser390 + Ser393) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-14399R-Cy3-100uL |
| Product Name | phospho-Dnmt3a (Ser390 + Ser393) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human Dnmt3a around the phosphorylation site of Ser390 + Ser393 |
| Modification Site | Ser390 + Ser393 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1788 |
| Swiss Prot | Q9Y6K1 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Dnmt3a (phospho S390 + S393); Dnmt3a (phospho Ser390 + Ser393); p-Dnmt3a (phospho S390 + S393); DNA (cytosine 5) methyltransferase 3 alpha; DNA (cytosine 5) methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaIIIA; DNM3A_HUMAN; DNMT 3a; DNMT; Dnmt3a; DNMT3A2; M.HsaIIIA; MCMT; OTTHUMP00000201149. |
| Background | Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is phospho-Dnmt3a (Ser390 + Ser393) Polyclonal Antibody, Cy3 Conjugated (bs-14399R-Cy3) validated for, and at what dilution?
bs-14399R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Dnmt3a (Ser390 + Ser393) Polyclonal Antibody, Cy3 Conjugated (bs-14399R-Cy3) react with?
Tested reactivity: Human.
How should bs-14399R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Dnmt3a (Ser390 + Ser393) Polyclonal Antibody, Cy3 Conjugated (bs-14399R-Cy3)?
KLH conjugated Synthesised phosphopeptide derived from human Dnmt3a around the phosphorylation site of Ser390 + Ser393
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-14399R-Cy3)?
Cy3 on bs-14399R-Cy3: excitation / emission 512,550nm/570,615nm.