DOLPP1 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14407R-BF594 |
| Product Name | DOLPP1 Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DOLPP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57171 |
| Swiss Prot | Q86YN1 |
| Subcellular location | Cytoplasm |
| Synonyms | 0610011H20Rik; AB030189; DOPP1_HUMAN; Dolichyldiphosphatase 1; Linked to Surfeit genes in Fugu rubripes 2; LSFR2; OTTHUMP00000022326; OTTHUMP00000196902. |
| Background | A similar gene has been characterized in mice and encodes dolichyl pyrophosphate (Dol-P-P) phosphatase. This protein dephosphorylates dolichyl pyrophosphate so that it may be re-utilized as a glycosyl carrier lipid by the oligosaccharyltransferase multisubunit complex in the ER. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DOLPP1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-14407R-BF594) validated for, and at what dilution?
bs-14407R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DOLPP1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-14407R-BF594) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-14407R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DOLPP1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-14407R-BF594)?
KLH conjugated synthetic peptide derived from human DOLPP1
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-14407R-BF594)?
AbBy Fluor-594 on bs-14407R-BF594: excitation / emission 590nm/617nm.