phospho-Dopamine Transporter (Thr53) Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14408R-HRP-100uL |
| Product Name | phospho-Dopamine Transporter (Thr53) Polyclonal Antibody, HRP Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Horse |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from mouse Dopamine Transporter around the phosphorylation site of Thr53 |
| Modification Site | Thr53 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 13162 |
| Swiss Prot | Q61327 |
| Subcellular location | Cell membrane |
| Synonyms | Dopamine Transporter (phospho T53); p-Dopamine Transporter (phospho T53); p-DAT1(phospho T53); DA transporter; DAT 1; DAT; SC6A3_RAT; DAT1; Dopamine transporter 1; Dopamine transporter; PKDYS; SC6A3_HUMAN; SLC6A3; Sodium dependent dopamine transporter; Sodium-dependent dopamine transporter; Solute carrier family 6 (neurotransmitter transporter dopamine), member 3; Solute carrier family 6 (neurotransmitter transporter), member 3; Solute carrier family 6 member 3; Variable number tandem repeat (VNTR). |
| Background | Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-Dopamine Transporter (Thr53) Polyclonal Antibody, HRP Conjugated (bs-14408R-HRP) validated for, and at what dilution?
bs-14408R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Dopamine Transporter (Thr53) Polyclonal Antibody, HRP Conjugated (bs-14408R-HRP) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Horse.
How should bs-14408R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Dopamine Transporter (Thr53) Polyclonal Antibody, HRP Conjugated (bs-14408R-HRP)?
KLH conjugated Synthesised phosphopeptide derived from mouse Dopamine Transporter around the phosphorylation site of Thr53