Free Shipping On Orders Over $1,000!

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

DPAGT1 Polyclonal Antibody

Mouse lung lysates probed with Rabbit Anti-DPAGT1 Polyclonal Antibody, Unconjugated (bs-14412R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.
Mouse lung lysates probed with Rabbit Anti-DPAGT1 Polyclonal Antibody, Unconjugated (bs-14412R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-DPAGT1 Polyclonal Antibody, Unconjugated (bs-14412R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-DPAGT1 Polyclonal Antibody, Unconjugated (bs-14412R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DPAGT1 Polyclonal Antibody, Unconjugated (bs-14412R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DPAGT1 Polyclonal Antibody, Unconjugated (bs-14412R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)
  • ICC

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-14412R
Product Name DPAGT1 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DPAGT1
Immunogen Range 301-408/408
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1798
Swiss Prot Q9H3H5
Subcellular location Cytoplasm
Synonyms GPT; ALG7; DGPT; G1PT; UAGT; UGAT; CDG1J; DPAGT; CDG-Ij; CMSTA2; DPAGT2; D11S366; UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase; GlcNAc-1-P transferase; N-acetylglucosamine-1-phosphate transferase; DPAGT1
Background Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Search our catalog of hundreds of thousands of products