TRAP1/HSP75 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-1446R-Cy3 |
| Product Name | TRAP1/HSP75 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HSP75 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10131 |
| Swiss Prot | Q12931 |
| Subcellular location | Cytoplasm |
| Synonyms | HSP75; HSP 75; HSP90L; TRAP-1; Heat shock protein 75 kDa, mitochondrial; TNFR-associated protein 1; Tumor necrosis factor type 1 receptor-associated protein; TRAP1 |
| Background | Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, most likely through stabilization of mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TRAP1/HSP75 Polyclonal Antibody, Cy3 Conjugated (bs-1446R-Cy3) validated for, and at what dilution?
bs-1446R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-1446R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TRAP1/HSP75 Polyclonal Antibody, Cy3 Conjugated (bs-1446R-Cy3)?
KLH conjugated synthetic peptide derived from human HSP75
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1446R-Cy3)?
Cy3 on bs-1446R-Cy3: excitation / emission 512,550nm/570,615nm.