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EAN57 Polyclonal Antibody, HRP Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-14481R-HRP
Product Name EAN57 Polyclonal Antibody, HRP Conjugated
Applications
ELISA IHC-P IHC-F
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Pig, Horse
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human EAN57
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 339669
Swiss Prot O43247
Subcellular location Nucleus
Synonyms C22orf33; cE81G9.2; Chromosome 22 open reading frame 33; Ean57; TEX33_HUMAN; Protein EAN57; Testis expressed sequence 33 protein; TEX33.
Background EAN57 is a 59 amino acid protein encoded by the C22orf33 protein. Chromosome 22 contains over 500 genes and about 49 million bases. Being the second smallest human chromosome, 22 contains a surprising variety of interesting genes. Phelan-McDermid syndrome, Neurofibromatosis type 2 and autism are associated with chromosome 22. A schizophrenia susceptibility locus has been identified on chromosome 22 and studies show that 22q11 deletion symptoms include a high incidence of schizophrenia. Translocations between chromosomes 9 and 22 may lead to the formation of the Philadelphia Chromosome and the subsequent production of the novel fusion protein, BCR-Abl, a potent cell proliferation activator found in several types of leukemia.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is EAN57 Polyclonal Antibody, HRP Conjugated (bs-14481R-HRP) validated for, and at what dilution?

bs-14481R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EAN57 Polyclonal Antibody, HRP Conjugated (bs-14481R-HRP) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse.

How should bs-14481R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise EAN57 Polyclonal Antibody, HRP Conjugated (bs-14481R-HRP)?

KLH conjugated synthetic peptide derived from human EAN57