EAP30 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14482R-FITC |
| Product Name | EAP30 Polyclonal Antibody, FITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EAP30 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11267 |
| Swiss Prot | Q96H20 |
| Subcellular location | Cytoplasm |
| Synonyms | Dot3; ELL associated protein of 30 kDa; ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; hVps22; snf8; SNF8 ESCRTII complex subunit homolog (S cerevisiae); SNF8_HUMAN; Vacuolar sorting protein SNF8; Vacuolar-sorting protein SNF8; VPS22. |
| Background | Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EAP30 Polyclonal Antibody, FITC Conjugated (bs-14482R-FITC) validated for, and at what dilution?
bs-14482R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EAP30 Polyclonal Antibody, FITC Conjugated (bs-14482R-FITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-14482R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EAP30 Polyclonal Antibody, FITC Conjugated (bs-14482R-FITC)?
KLH conjugated synthetic peptide derived from human EAP30
What are the excitation and emission wavelengths of the FITC conjugate (bs-14482R-FITC)?
FITC on bs-14482R-FITC: excitation / emission 494nm/518nm.