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Prohibitin Polyclonal Antibody

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-Prohibitin Polyclonal Antibody, Unconjugated (bs-1449R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-Prohibitin Polyclonal Antibody, Unconjugated (bs-1449R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse heart lysates probed with Prohibitin Polyclonal Antibody, Unconjugated (bs-1449R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse heart lysates probed with Prohibitin Polyclonal Antibody, Unconjugated (bs-1449R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • Others

Predicted Reactivity

  • Dog
  • Horse
Overview
Catalog # bs-1449R
Product Name Prohibitin Polyclonal Antibody
Applications WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Rat, Others
Predicted Reactivity Dog, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Prohibitin
Immunogen Range 171-272/272
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5245
Swiss Prot P35232
Subcellular location Cytoplasm
Synonyms PHB1; HEL-215; HEL-S-54e; Prohibitin; PHB
Background Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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