EDEM3 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14502R-BF647 |
| Product Name | EDEM3 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EDEM3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 80267 |
| Swiss Prot | Q9BZQ6 |
| Subcellular location | Cytoplasm |
| Synonyms | C1orf22; EDEM 3; edem3; EDEM3_HUMAN; ER degradation enhancer, mannosidase alpha like 3; ER degradation enhancing alpha mannosidase like 3; ER degradation enhancing alpha mannosidase like 3; ER degradation-enhancing alpha-mannosidase-like 3. |
| Background | Quality control in the endoplasmic reticulum (ER) ensures that only properly folded proteins are retained in the cell through recognition and degradation of misfolded or unassembled proteins. EDEM3 belongs to a group of proteins that accelerate degradation of misfolded glycoproteins in the ER (Hirao et al., 2006 [PubMed 16431915]).[supplied by OMIM, Mar 2008] |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EDEM3 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-14502R-BF647) validated for, and at what dilution?
bs-14502R-BF647 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EDEM3 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-14502R-BF647) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Pig, Horse, Chicken.
How should bs-14502R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EDEM3 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-14502R-BF647)?
KLH conjugated synthetic peptide derived from human EDEM3
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-14502R-BF647)?
AbBy Fluor-647 on bs-14502R-BF647: excitation / emission 650nm/665nm.