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EDEM3 Polyclonal Antibody, Biotin Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-14502R-Biotin
Product Name EDEM3 Polyclonal Antibody, Biotin Conjugated
Applications
ELISA IHC-P IHC-F
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human EDEM3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 80267
Swiss Prot Q9BZQ6
Subcellular location Cytoplasm
Synonyms C1orf22; EDEM 3; edem3; EDEM3_HUMAN; ER degradation enhancer, mannosidase alpha like 3; ER degradation enhancing alpha mannosidase like 3; ER degradation enhancing alpha mannosidase like 3; ER degradation-enhancing alpha-mannosidase-like 3.
Background Quality control in the endoplasmic reticulum (ER) ensures that only properly folded proteins are retained in the cell through recognition and degradation of misfolded or unassembled proteins. EDEM3 belongs to a group of proteins that accelerate degradation of misfolded glycoproteins in the ER (Hirao et al., 2006 [PubMed 16431915]).[supplied by OMIM, Mar 2008]
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is EDEM3 Polyclonal Antibody, Biotin Conjugated (bs-14502R-Biotin) validated for, and at what dilution?

bs-14502R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EDEM3 Polyclonal Antibody, Biotin Conjugated (bs-14502R-Biotin) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Pig, Horse, Chicken.

How should bs-14502R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise EDEM3 Polyclonal Antibody, Biotin Conjugated (bs-14502R-Biotin)?

KLH conjugated synthetic peptide derived from human EDEM3