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EDEM3 Polyclonal Antibody, PerCP Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-14502R-PerCP
Product Name EDEM3 Polyclonal Antibody, PerCP Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human EDEM3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 80267
Swiss Prot Q9BZQ6
Subcellular location Cytoplasm
Synonyms C1orf22; EDEM 3; edem3; EDEM3_HUMAN; ER degradation enhancer, mannosidase alpha like 3; ER degradation enhancing alpha mannosidase like 3; ER degradation enhancing alpha mannosidase like 3; ER degradation-enhancing alpha-mannosidase-like 3.
Background Quality control in the endoplasmic reticulum (ER) ensures that only properly folded proteins are retained in the cell through recognition and degradation of misfolded or unassembled proteins. EDEM3 belongs to a group of proteins that accelerate degradation of misfolded glycoproteins in the ER (Hirao et al., 2006 [PubMed 16431915]).[supplied by OMIM, Mar 2008]
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is EDEM3 Polyclonal Antibody, PerCP Conjugated (bs-14502R-PerCP) validated for, and at what dilution?

bs-14502R-PerCP is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EDEM3 Polyclonal Antibody, PerCP Conjugated (bs-14502R-PerCP) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Pig, Horse, Chicken.

How should bs-14502R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise EDEM3 Polyclonal Antibody, PerCP Conjugated (bs-14502R-PerCP)?

KLH conjugated synthetic peptide derived from human EDEM3

What are the excitation and emission wavelengths of the PerCP conjugate (bs-14502R-PerCP)?

PerCP on bs-14502R-PerCP: excitation / emission 482nm/677nm.