phospho-EDG1 (Thr236) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14504R-Cy7 |
| Product Name | phospho-EDG1 (Thr236) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human EDG1 around the phosphorylation site of Thr236 |
| Modification Site | Thr236 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1901 |
| Swiss Prot | P21453 |
| Subcellular location | Cell membrane |
| Synonyms | EDG1 (phospho T236); p-EDG1 (phospho T236); CD363; CHEDG 1; CHEDG1; D1S3362; ECGF 1; ECGF1; EDG 1; EDG1; endothelial differentiation G protein coupled receptor 1; Endothelial differentiation G-protein coupled receptor 1; Endothelial differentiation sphingolipid G protein coupled receptor 1; FLJ58121; G protein coupled sphingolipid receptor; g protein-coupled receptor edg-1; S1P receptor 1; S1P receptor Edg 1; S1P receptor Edg-1; S1P receptor Edg1; S1P(1) receptor; S1P1; s1pr1; S1PR1_HUMAN; sphingolipid g-protein-coupled receptor 1; Sphingosine 1 phosphate receptor Edg 1; Sphingosine 1 phosphate receptor EDG1; sphingosine 1- phosphate receptor 1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; Sphingosine 1phosphate receptor type 1 S1P1. |
| Background | This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-EDG1 (Thr236) Polyclonal Antibody, Cy7 Conjugated (bs-14504R-Cy7) validated for, and at what dilution?
bs-14504R-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-EDG1 (Thr236) Polyclonal Antibody, Cy7 Conjugated (bs-14504R-Cy7) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Pig, Rabbit.
How should bs-14504R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-EDG1 (Thr236) Polyclonal Antibody, Cy7 Conjugated (bs-14504R-Cy7)?
KLH conjugated synthesised phosphopeptide derived from human EDG1 around the phosphorylation site of Thr236
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-14504R-Cy7)?
Cy7 on bs-14504R-Cy7: excitation / emission 743nm/767nm.