EEFSEC Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14505R-BF594 |
| Product Name | EEFSEC Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EEFSEC |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 60678 |
| Swiss Prot | P57772 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | EFSEC; SELB_HUMAN_ Elongation factor for selenoprotein translation; Elongation factor sec; Eukaryotic elongation factor, selenocysteine tRNA specific; SELB; Selenocysteine specific elongation factor. |
| Background | EEFSEC is a 596 amino acid protein that localizes to both the nucleus and the cytoplasm and belongs to the GTP-binding elongation factor family. Functioning as a translation factor, SELB binds GTP and GDP and is necessary for the incorporation of selenocysteine into target proteins. The gene encoding SELB maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EEFSEC Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-14505R-BF594) validated for, and at what dilution?
bs-14505R-BF594 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EEFSEC Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-14505R-BF594) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Rabbit.
How should bs-14505R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EEFSEC Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-14505R-BF594)?
KLH conjugated synthetic peptide derived from human EEFSEC
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-14505R-BF594)?
AbBy Fluor-594 on bs-14505R-BF594: excitation / emission 590nm/617nm.