phospho-EEF1A2_Ser358_ Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14508R-APC |
| Product Name | phospho-EEF1A2_Ser358_ Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human EEF1A2 around the phosphorylation site of Ser358 |
| Modification Site | Ser358 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1917 |
| Swiss Prot | Q05639 |
| Subcellular location | Nucleus |
| Synonyms | EEF1A2 (phospho S358);p-EEF1A2 (phospho S358); eEF1A 2; eEF1A-2; Eef1a2; EEF1AL; EF 1 alpha 2; EF-1-alpha-2; EF1A; EF1A2_HUMAN; Elongation factor 1 A 2; Elongation factor 1 alpha; Elongation factor 1 alpha 2; Elongation factor 1-alpha 2; Eukaryotic elongation factor 1 A-2; Eukaryotic translation elongation factor 1 alpha 2; HS1; OTTHUMP00000031776; Statin like; Statin S1; Statin-S1; STN; STNL. |
| Background | This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-EEF1A2_Ser358_ Polyclonal Antibody, APC Conjugated (bs-14508R-APC) validated for, and at what dilution?
bs-14508R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-EEF1A2_Ser358_ Polyclonal Antibody, APC Conjugated (bs-14508R-APC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-14508R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-EEF1A2_Ser358_ Polyclonal Antibody, APC Conjugated (bs-14508R-APC)?
KLH conjugated Synthesised phosphopeptide derived from human EEF1A2 around the phosphorylation site of Ser358
What are the excitation and emission wavelengths of the APC conjugate (bs-14508R-APC)?
APC on bs-14508R-APC: excitation / emission 650nm/660nm.