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phospho-EEF1A2_Ser358_ Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bs-14508R-PE
Product Name phospho-EEF1A2_Ser358_ Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated Synthesised phosphopeptide derived from human EEF1A2 around the phosphorylation site of Ser358
Modification Site Ser358
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1917
Swiss Prot Q05639
Subcellular location Nucleus
Synonyms EEF1A2 (phospho S358);p-EEF1A2 (phospho S358); eEF1A 2; eEF1A-2; Eef1a2; EEF1AL; EF 1 alpha 2; EF-1-alpha-2; EF1A; EF1A2_HUMAN; Elongation factor 1 A 2; Elongation factor 1 alpha; Elongation factor 1 alpha 2; Elongation factor 1-alpha 2; Eukaryotic elongation factor 1 A-2; Eukaryotic translation elongation factor 1 alpha 2; HS1; OTTHUMP00000031776; Statin like; Statin S1; Statin-S1; STN; STNL.
Background This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is phospho-EEF1A2_Ser358_ Polyclonal Antibody, PE Conjugated (bs-14508R-PE) validated for, and at what dilution?

bs-14508R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-EEF1A2_Ser358_ Polyclonal Antibody, PE Conjugated (bs-14508R-PE) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bs-14508R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-EEF1A2_Ser358_ Polyclonal Antibody, PE Conjugated (bs-14508R-PE)?

KLH conjugated Synthesised phosphopeptide derived from human EEF1A2 around the phosphorylation site of Ser358

What are the excitation and emission wavelengths of the PE conjugate (bs-14508R-PE)?

PE on bs-14508R-PE: excitation / emission 496,564nm/578nm.