eIF2B1 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14535R-100uL |
| Product Name | eIF2B1 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human eIF2B1 |
| Immunogen Range | 51-150/305 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1967 |
| Swiss Prot | Q14232 |
| Subcellular location | Cytoplasm |
| Synonyms | 26kDa; D5Ertd406e; EI2BA; eIF 2a; EIF 2B; eIF 2B GDP GTP exchange factor subunit alpha; EIF 2Balpha; EIF2B alpha; EIF2B; EIF2B1; EIF2BA; eukaryotic translation initiation factor 2B subunit 1 alpha; Eukaryotic translation initiation factor 2B, alpha; eukaryotic translation initiation factor 2B, subunit 1 (alpha, 26kD); Eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa; MGC117409; MGC125868; EI2BA_HUMAN; MGC125869; MGC6458; Translation initiation factor eIF2B subunit alpha; zgc:86660. |
| Background | This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Oct 2009] |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is eIF2B1 Polyclonal Antibody (bs-14535R) validated for, and at what dilution?
bs-14535R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eIF2B1 Polyclonal Antibody (bs-14535R) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-14535R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eIF2B1 Polyclonal Antibody (bs-14535R)?
KLH conjugated synthetic peptide derived from human eIF2B1 Immunogen range: 51-150/305.