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EIF2B3 Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Horse
  • Rabbit
Overview
Catalog # bs-14537R-RBITC-100uL
Product Name EIF2B3 Polyclonal Antibody, RBITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Horse, Rabbit
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human EIF2B3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8891
Swiss Prot Q9NR50
Subcellular location Cytoplasm
Synonyms EIF-2B; EIF2Bgamma; Translation initiation factor eIF-2B subunit gamma; eIF-2B GDP-GTP exchange factor subunit gamma; EIF2B3
Background Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is EIF2B3 Polyclonal Antibody, RBITC Conjugated (bs-14537R-RBITC) validated for, and at what dilution?

bs-14537R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EIF2B3 Polyclonal Antibody, RBITC Conjugated (bs-14537R-RBITC) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Horse, Rabbit.

How should bs-14537R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise EIF2B3 Polyclonal Antibody, RBITC Conjugated (bs-14537R-RBITC)?

KLH conjugated synthetic peptide derived from human EIF2B3

What are the excitation and emission wavelengths of the RBITC conjugate (bs-14537R-RBITC)?

RBITC on bs-14537R-RBITC: excitation / emission 540nm/625nm.