EIF3S2 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-14544r-bf750 |
| Product Name | EIF3S2 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EIF3S2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | Q13347 |
| Swiss Prot | 8668 |
| Synonyms | eIF-3-beta; eIF3 beta; eIF3 p36; eIF3-beta; eIF3-p36; eIF3i; EIF3I_HUMAN; EIF3S2; EIF3S2, formerly; Eukaryotic translation initiation factor 3 subunit 2; Eukaryotic translation initiation factor 3 subunit 2 beta; Eukaryotic translation initiation factor 3 subunit I; eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD; eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa; eukaryotic translation initiation factor 3, subunit 2, formerly; eukaryotic translation initiation factor 3, subunit I; Predicted protein of HQ2242; PRO2242; TGF beta receptor interacting protein 1; TGF-beta receptor-interacting protein 1; TGFbeta receptor interacting protein 1; TGFbeta receptor-interacting protein 1; TRIP 1; TRIP-1; TRIP1. |
| Background | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that lead to 80S ribosomal assembly and, ultimately, translation. The eukaryotic initiation factor-3 (eIF3) scaffolding structure is the largest of the eIF complexes and includes eIF3 alpha, eIF3 beta, eIF3 gamma, eIF3 delta, eIF3 epsilon, eIF3 omega, eIF3 eta, all of which function to control the assembly of the 40S ribosomal subunit. Association of eIF3 proteins with the 40S ribosomal subunit stabilizes eIF2-GTP-Met-tRNAiMet complex association and mRNA binding, and promotes dissociation of 80S ribosomes into 40S and 60S subunits, thereby promoting the assembly of the pre-initiation complex. Overexpression of eIF3 proteins is common in several cancers, suggesting a role for eIF3 proteins in tumorigenesis. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is EIF3S2 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-14544R-BF750) validated for, and at what dilution?
bs-14544R-BF750 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EIF3S2 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-14544R-BF750) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-14544R-BF750 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EIF3S2 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-14544R-BF750)?
KLH conjugated synthetic peptide derived from human EIF3S2
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-14544R-BF750)?
AbBy Fluor-750 on bs-14544R-BF750: excitation / emission 749nm/775nm.