eIF4A1 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14547R-BF488 |
| Product Name | eIF4A1 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Sheep, Horse, African Green Monkey |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human eIF4A1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1973 |
| Swiss Prot | P60842 |
| Subcellular location | Extracellular, Cytoplasm, Secreted |
| Synonyms | ATP-dependent RNA helicase eIF4A-1; DDX2; DDX2A; eIF 4A I; eIF-4A-I; EIF4A; eIF4A I; eIF4A-I; EIF4A1; Eukaryotic initiation factor 4A-I; Eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A, isoform 1; Eukaryotic translation initiation factor 4A1; IF4A1_HUMAN. |
| Background | Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a 5-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5 cap, eIF4A(I,II) are bidirectional RNA helicases, and eIF4G(I,II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3 and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2 |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is eIF4A1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14547R-BF488) validated for, and at what dilution?
bs-14547R-BF488 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eIF4A1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14547R-BF488) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Sheep, Horse, African Green Monkey.
How should bs-14547R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eIF4A1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14547R-BF488)?
KLH conjugated synthetic peptide derived from human eIF4A1
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-14547R-BF488)?
AbBy Fluor-488 on bs-14547R-BF488: excitation / emission 499nm/519nm.