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eIF4H Polyclonal Antibody, PerCP Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
  • Orangutan
Overview
Catalog # bs-14553R-PerCP
Product Name eIF4H Polyclonal Antibody, PerCP Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, Orangutan
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human eIF4H
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7458
Swiss Prot Q15056
Subcellular location Cytoplasm
Synonyms WSCR1; WBSCR1; eIF-4H; Eukaryotic translation initiation factor 4H; Williams-Beuren syndrome chromosomal region 1 protein; EIF4H; KIAA0038
Background Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is eIF4H Polyclonal Antibody, PerCP Conjugated (bs-14553R-PerCP) validated for, and at what dilution?

bs-14553R-PerCP is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does eIF4H Polyclonal Antibody, PerCP Conjugated (bs-14553R-PerCP) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, Orangutan.

How should bs-14553R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise eIF4H Polyclonal Antibody, PerCP Conjugated (bs-14553R-PerCP)?

KLH conjugated synthetic peptide derived from human eIF4H

What are the excitation and emission wavelengths of the PerCP conjugate (bs-14553R-PerCP)?

PerCP on bs-14553R-PerCP: excitation / emission 482nm/677nm.