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eIF4H Polyclonal Antibody, PerCP-Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
  • Orangutan
Overview
Catalog # bs-14553r-percp-cy7-100uL
Product Name eIF4H Polyclonal Antibody, PerCP-Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, Orangutan
Specifications
Conjugation PerCP-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human eIF4H
Immunogen Range 31-130/248
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7458
Swiss Prot Q15056
Subcellular location Cytoplasm
Synonyms WSCR1; WBSCR1; eIF-4H; Eukaryotic translation initiation factor 4H; Williams-Beuren syndrome chromosomal region 1 protein; EIF4H; KIAA0038
Background Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is eIF4H Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-14553R-PerCP-Cy7) validated for, and at what dilution?

bs-14553R-PerCP-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does eIF4H Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-14553R-PerCP-Cy7) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, Orangutan.

How should bs-14553R-PerCP-Cy7 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise eIF4H Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-14553R-PerCP-Cy7)?

KLH conjugated synthetic peptide derived from human eIF4H Immunogen range: 31-130/248.

What are the excitation and emission wavelengths of the PerCP-Cy7 conjugate (bs-14553R-PerCP-Cy7)?

PerCP-Cy7 on bs-14553R-PerCP-Cy7: excitation / emission 482nm/780nm.

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