eIF4ENIF1 Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14561R-PerCP |
| Product Name | eIF4ENIF1 Polyclonal Antibody, PerCP Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Horse, Pig, Rabbit, Chimpanzee, Rhesus monkey, Gorilla |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human eIF4ENIF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 56478 |
| Swiss Prot | Q9NRA8 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | 4E-T; Clast4; Eukaryotic translation initiation factor 4E transporter; eIF4E transporter; Eukaryotic translation initiation factor 4E nuclear import factor 1; EIF4ENIF1 |
| Background | Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is eIF4ENIF1 Polyclonal Antibody, PerCP Conjugated (bs-14561R-PerCP) validated for, and at what dilution?
bs-14561R-PerCP is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eIF4ENIF1 Polyclonal Antibody, PerCP Conjugated (bs-14561R-PerCP) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Horse, Pig, Rabbit, Chimpanzee, Rhesus monkey, Gorilla.
How should bs-14561R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eIF4ENIF1 Polyclonal Antibody, PerCP Conjugated (bs-14561R-PerCP)?
KLH conjugated synthetic peptide derived from human eIF4ENIF1
What are the excitation and emission wavelengths of the PerCP conjugate (bs-14561R-PerCP)?
PerCP on bs-14561R-PerCP: excitation / emission 482nm/677nm.