EMID1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EMID1 Polyclonal Antibody, Unconjugated (bs-14581R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EMID1 Polyclonal Antibody, Unconjugated (bs-14581R) at 1:400 overnight at 4\u00b0C, DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow

Overview
Catalog #	bs-14581R
Product Name	EMID1 Polyclonal Antibody
Applications	ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human EMID1
Immunogen Range	351-441/441
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	129080
Swiss Prot	Q96A84
Subcellular location	Cytoplasm
Synonyms	AW122071; CO 5; CTA-984G1.2; EMI domain containing 1; EMI domain containing protein 1; EMI domain-containing protein 1; EMI5; EMID 1; Emid1; EMID1_HUMAN; Emilin and multimerin domain containing protein 1; Emilin and multimerin domain-containing protein 1; EMU1; hEmu1; MGC50657; OTTMUSP00000005297; Protein Emu1; Putative emu1; RGD1565846; RP23-338J18.3.
Background	Chromosome 22 contains over 500 genes and about 49 million bases. Being the second smallest human chromosome, 22 contains a surprising variety of interesting genes. Phelan-McDermid syndrome, Neurofibromatosis type 2 and autism are associated with chromosome 22. A schizophrenia susceptibility locus has been identified on chromosome 22 and studies show that 22q11 deletion symptoms include a high incidence of schizophrenia. Translocations between chromosomes 9 and 22 may lead to the formation of the Philadelphia Chromosome and the subsequent production of the novel fusion protein, BCR-Abl, a potent cell proliferation activator found in several types of leukemia.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200