IL6ST/CD130 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-IL-6R Beta\/CD130\/gp130 Polyclonal Antibody, Unconjugated (bs-1459R) followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-IL-6R Beta\/CD130\/gp130 Polyclonal Antibody, Unconjugated (bs-1459R) followed by conjugation to the secondary antibody and DAB staining

$Mouse lung lysates probed with IL-6R Beta\/CD130\/gp130 Antibody, Unconjugated (bs-1459R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.$

Mouse lung lysates probed with IL-6R Beta\/CD130\/gp130 Antibody, Unconjugated (bs-1459R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

Human Raji cells probed with IL-6R beta Polyclonal Antibody, Unconjugated (bs-1459R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

$Paraformaldehyde-fixed, paraffin-embedded Human Colon Carcinoma; Antigen retrieval by boiling in sodium citrate buffer(0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-6R Beta\/CD130\/gp130 Antibody Polyclonal Antibody, Unconjugated (bs-1459R) at 1:300 overnight at 4\u00b0C. A Goat Anti-Rabbit IgG, FITC Conjugated secondary antibody (bs-0295G-FITC) was used for 40 mins at 37\u00b0C. DAPI (5ug\/ml, blue) was used for nuclei staining.$

Paraformaldehyde-fixed, paraffin-embedded Human Colon Carcinoma; Antigen retrieval by boiling in sodium citrate buffer(0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-6R Beta\/CD130\/gp130 Antibody Polyclonal Antibody, Unconjugated (bs-1459R) at 1:300 overnight at 4\u00b0C. A Goat Anti-Rabbit IgG, FITC Conjugated secondary antibody (bs-0295G-FITC) was used for 40 mins at 37\u00b0C. DAPI (5ug\/ml, blue) was used for nuclei staining.

$Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nIL-6R Beta\/CD130\/gp130 Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nIL-6R Beta\/CD130\/gp130 Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nIL-6R Beta\/CD130\/gp130 Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nIL-6R Beta\/CD130\/gp130 Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-1459R
Product Name	IL6ST/CD130 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse IL6R beta
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	16195
Swiss Prot	Q00560
Subcellular location	Secreted, Cell membrane
Synonyms	Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; Il6st
Background	Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200