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ERp57 Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Chicken
  • Rabbit
Overview
Catalog # bs-14629R-PerCP
Product Name ERp57 Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This antibody may have slight cross reactivity with ZFYVE26 and DCLRE1C due to partial sequence similarity
Reactivity Human, Mouse, Rat
Predicted Reactivity Chicken, Rabbit
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ERp57
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2923
Swiss Prot P30101
Subcellular location Cytoplasm
Synonyms P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n; Protein disulfide-isomerase A3; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; Endoplasmic reticulum resident protein 60; ER protein 60; PDIA3
Background Catalyzes the rearrangement of -S-S- bonds in proteins.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ERp57 Polyclonal Antibody, PerCP Conjugated (bs-14629R-PerCP) validated for, and at what dilution?

bs-14629R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ERp57 Polyclonal Antibody, PerCP Conjugated (bs-14629R-PerCP) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Chicken, Rabbit.

How should bs-14629R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ERp57 Polyclonal Antibody, PerCP Conjugated (bs-14629R-PerCP)?

KLH conjugated synthetic peptide derived from human ERp57

What are the excitation and emission wavelengths of the PerCP conjugate (bs-14629R-PerCP)?

PerCP on bs-14629R-PerCP: excitation / emission 482nm/677nm.