EXOC1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14657R-RBITC |
| Product Name | EXOC1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EXOC1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55763 |
| Swiss Prot | Q9NV70 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | BM-012; exoc1; EXOC1_HUMAN; Exocyst complex component 1; Exocyst complex component Sec3; FLJ10893; SEC3; SEC3 like 1; SEC3L1; SEC3P. |
| Background | Exocytosis is crucial in membrane trafficking and it mediates hormone and neurotransmitter secretion out of the cell, as well as the incorporation of membrane proteins and lipids to the plasma membrane. It is crucial for cell-cell communication, cell growth and cell polarity. The exocyst complex is a multi-protein complex that consists of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84, and is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Exo70, also known as EXOC7 (exocyst complex component 7), EXOC1 or 2-5-3p, is a 735 amino acid peripheral membrane protein that is a component of the exocyst complex. Localized to the cytoplasm and the cell membrane, Exo70 plays an essential role in the docking of exocystic vesicles to target sites on the plasma membrane and, specifically, may be involved in insulin-induced protein docking within the cell. Four isoforms of Exo70 are expressed due to alternative splicing events. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EXOC1 Polyclonal Antibody, RBITC Conjugated (bs-14657R-RBITC) validated for, and at what dilution?
bs-14657R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EXOC1 Polyclonal Antibody, RBITC Conjugated (bs-14657R-RBITC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Pig, Horse.
How should bs-14657R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EXOC1 Polyclonal Antibody, RBITC Conjugated (bs-14657R-RBITC)?
KLH conjugated synthetic peptide derived from human EXOC1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-14657R-RBITC)?
RBITC on bs-14657R-RBITC: excitation / emission 540nm/625nm.