GRP75 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Lane 1: MCF-7 cell lysates; Lane 2: HepG2 cell lysates probed with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Liver tissue lysates; Lane 5: Rat Testis tissue lysates; Lane 6: Rat Cerebrum tissue lysates; Lane 7: Rat Pancreas tissue lysates; Lane 8: Human K562 cell lysates; Lane 9: Human MCF-7 cell lysates; Lane 10: Human Jurkat cell lysates; Lane 11: Human HeLa cell lysates; Lane 12: Human HepG2 cell lysates probed with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse Cerebrum tissue lysates; Lane 4: Rat Liver tissue lysates; Lane 5: Rat Testis tissue lysates; Lane 6: Rat Cerebrum tissue lysates; Lane 7: Rat Pancreas tissue lysates; Lane 8: Human K562 cell lysates; Lane 9: Human MCF-7 cell lysates; Lane 10: Human Jurkat cell lysates; Lane 11: Human HeLa cell lysates; Lane 12: Human HepG2 cell lysates probed with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-1469R
Product Name	GRP75 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GRP75
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3313
Swiss Prot	P38646
Subcellular location	Cytoplasm, Nucleus
Synonyms	CSA; MOT; MOT2; CRP40; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; HEL-S-124m; Stress-70 protein, mitochondrial; 75 kDa glucose-regulated protein; Heat shock 70 kDa protein 9; Mortalin; Peptide-binding protein 74; HSPA9; mt-HSP70
Background	Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200