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WTX Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Sheep
  • Horse
Overview
Catalog # bs-14710R-APC-Cy7-100uL
Product Name WTX Polyclonal Antibody, APC-Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Sheep, Horse
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human WTX
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 139285
Swiss Prot Q5JTC6
Subcellular location Cytoplasm, Cell membrane
Synonyms FAM123B; AMER1; FAM 123B; AMER1_HUMAN; Family with sequence similarity 123B; FLJ39827; OSCS; Protein FAM123B; RP11 403E24.2; Wilms tumor gene on the X chromosome protein; Wilms tumor on the X; WTX.
Background FAM123B enhances trancription activation by WT1. It also promotes CTNNB1 ubiquitination and degradation and antagonizes Wnt and CTNNB1 signaling. FAM123B is involved in kidney development.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is WTX Polyclonal Antibody, APC-Cy7 Conjugated (bs-14710R-APC-Cy7) validated for, and at what dilution?

bs-14710R-APC-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does WTX Polyclonal Antibody, APC-Cy7 Conjugated (bs-14710R-APC-Cy7) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Horse.

How should bs-14710R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise WTX Polyclonal Antibody, APC-Cy7 Conjugated (bs-14710R-APC-Cy7)?

KLH conjugated synthetic peptide derived from human WTX

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-14710R-APC-Cy7)?

APC-Cy7 on bs-14710R-APC-Cy7: excitation / emission 650nm/780nm.