Occludin Polyclonal Antibody

$Image provided by the Independent Validation Program, badge number 029632:Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-OCLN\/Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:200 followed by conjugation to the secondary antibody and DAPI staining$

Image provided by the Independent Validation Program, badge number 029632:Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-OCLN\/Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:200 followed by conjugation to the secondary antibody and DAPI staining

$Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37\u02daC.$

Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37\u02daC.

293T cells probed with Occludin Polyclonal Antibody, unconjugated (bs-1495R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)

$Mouse Kidney lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37\u02daC.$

Mouse Kidney lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37\u02daC.

$Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human Hela cell lysates probed with Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human Hela cell lysates probed with Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat
Pig

Overview
Catalog #	bs-1495R
Product Name	Occludin Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse Occludin
Immunogen Range	480-520/521
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Gene ID	18260
Swiss Prot	Q61146
Subcellular location	Cytoplasm
Synonyms	Ocl; AI53564; Occludin; Ocln
Background	May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
Application Dilution
WB	1:300-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200

Applications

Reactivity

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