PIEZO1 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-14991R-BF488 |
| Product Name | PIEZO1 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PIEZO1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9780 |
| Swiss Prot | Q92508 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Piezo-type mechanosensitive ion channel component 1; Piezo-type mechanosensitive ion channel component 2; PIEZO1; PIEZO2; Mib; C18orf30; C18orf58; FAM38B; FAM38A; KIAA0233 |
| Background | Piezos are large transmembrane proteins conserved among various species, all having between 24 and 36 predicted transmembrane domains. 'Piezo' comes from the Greek 'piesi,' meaning 'pressure.' The FAM38A gene encodes PIEZO1, a protein that induces mechanically activated (MA) currents in various cell types. PIEZO2 Component of a mechanosensitive channel required for rapidly adapting mechanically activated (MA) currents. Required for Merkel-cell mechanotransduction. Plays a major role in light-touch mechanosensation |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PIEZO1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14991R-BF488) validated for, and at what dilution?
bs-14991R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PIEZO1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14991R-BF488) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep.
How should bs-14991R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PIEZO1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-14991R-BF488)?
KLH conjugated synthetic peptide derived from human PIEZO1
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-14991R-BF488)?
AbBy Fluor-488 on bs-14991R-BF488: excitation / emission 499nm/519nm.