C1orf135 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-15019R-PerCP-Cy5.5-100uL |
| Product Name | C1orf135 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human C1orf135 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79000 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | C1orf135; CA135_HUMAN; Uncharacterized protein C1orf135. |
| Background | Chromosome 1 is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1. Notably, the rare aging disease Hutchinson-Gilford progeria is associated with the LMNA gene which encodes lamin A. When defective, the LMNA gene product can build up in the nucleus and cause characteristic nuclear blebs. The mechanism of rapidly enhanced aging is unclear and is a topic of continuing exploration. The MUTYH gene is located on chromosome 1 and is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome are also associated with chromosome 1. A breakpoint has been identified in 1q which disrupts the DISC1 gene and is linked to schizophrenia. Aberrations in chromosome 1 are found in a variety of cancers including head and neck cancer, malignant melanoma and multiple myeloma. The C1orf135 gene product has been provisionally designated C1orf135 pending further characterization. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is C1orf135 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-15019R-PERCP-CY5-5) validated for, and at what dilution?
BS-15019R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-15019R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise C1orf135 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-15019R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human C1orf135
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-15019R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-15019R-PERCP-CY5-5: excitation / emission 482nm/694nm.