AAK1 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1507R-Cy7 |
| Product Name | AAK1 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human AAK1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 22848 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | AAK 1; Adaptor associated kinase 1; Aak1; AAK1_HUMAN; Adaptor-associated kinase 1; AP2-associated protein kinase 1; DKFZp686F03202; AP2 associated kinase 1;AP2 associated protein kinase 1; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; KIAA1048; MGC138170; MGC164568; MGC164570. |
| Background | AAK1 phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). It may play a role in regulating aspects of clathrin-mediated endocytosis. [SUBCELLULAR LOCATION] Membrane; Peripheral membrane protein (By similarity). Cell membrane; Peripheral membrane protein (By similarity). Membrane, clathrin-coated pit (By similarity). Note=Active when found in clathrin-coated pits at the plasma membrane (By similarity). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is AAK1 Polyclonal Antibody, Cy7 Conjugated (bs-1507R-Cy7) validated for, and at what dilution?
bs-1507R-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AAK1 Polyclonal Antibody, Cy7 Conjugated (bs-1507R-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-1507R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AAK1 Polyclonal Antibody, Cy7 Conjugated (bs-1507R-Cy7)?
KLH conjugated synthetic peptide derived from human AAK1
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-1507R-Cy7)?
Cy7 on bs-1507R-Cy7: excitation / emission 743nm/767nm.