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BMPR1A Polyclonal Antibody

L1 rat brain lysates L2 mouse intestine lysates probed with Anti BMPR1A\/CD292 Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 60kD. Observed band size:60kD.\\n
L1 rat brain lysates L2 mouse intestine lysates probed with Anti BMPR1A\/CD292 Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 60kD. Observed band size:60kD.\\n
Lane 1: Mouse Skin lysates probed with BMPR1A Polyclonal Antibody, Unconjugated (bs-1509R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Skin lysates probed with BMPR1A Polyclonal Antibody, Unconjugated (bs-1509R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Placenta lysates probed with BMPR1A Polyclonal Antibody, Unconjugated (bs-1509R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Placenta lysates probed with BMPR1A Polyclonal Antibody, Unconjugated (bs-1509R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Large intestine lysates probed with BMPR1A Polyclonal Antibody, Unconjugated (bs-1509R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Large intestine lysates probed with BMPR1A Polyclonal Antibody, Unconjugated (bs-1509R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Du145 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human U2os cell lysates ; Lane 5: Human SH-SY5Y cell lysates probed with BMPR1A Polyclonal Antibody, Unconjugated (bs-1509R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Du145 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human U2os cell lysates ; Lane 5: Human SH-SY5Y cell lysates probed with BMPR1A Polyclonal Antibody, Unconjugated (bs-1509R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat bone); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat bone); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human prostate ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human prostate ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
A549 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BMPR1A Polyclonal Antibody(bs-1509R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A549 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BMPR1A Polyclonal Antibody(bs-1509R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human 293T cell lysates; Lane 2: Human DU145 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human U251 cell lysates; Lane 6: Human A549 cell lysates probed with BMPR1A Polyclonal Antibody, Unconjugated (bs-1509R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human 293T cell lysates; Lane 2: Human DU145 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human U251 cell lysates; Lane 6: Human A549 cell lysates probed with BMPR1A Polyclonal Antibody, Unconjugated (bs-1509R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bs-1509R
Product Name BMPR1A Polyclonal Antibody
Applications WB, IHC-P, IHC-F, IF, Flow-Cyt
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BMPR-1A
Clonality Polyclonal
Clone # #REF!
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 657
Swiss Prot P36894
Subcellular location Cell membrane
Synonyms ALK3; SKR5; CD292; ACVRLK3; 10q23del; Bone morphogenetic protein receptor type-1A; BMP type-1A receptor; BMPR-1A; Activin receptor-like kinase 3; ALK-3; Serine/threonine-protein kinase receptor R5; BMPR1A
Background On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP-2 and BMP-4.
Application Dilution
WB 1:300-5000
IHC-P ELISA1:500-1000
IHC-F FCM1:20-100
IF IHC-P1:200-400
Flow-Cyt

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