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C20orf96 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
Overview
Catalog # bs-15120R-Cy3
Product Name C20orf96 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human C20orf96
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Nucleus
Synonyms Chromosome 20 open reading frame 96; dJ1103G7.2; Uncharacterized protein C20orf96; CT096_HUMAN.
Background Representing about 2% of human DNA, chromosome 20 consists of approximately 63 million bases and 600 genes. Chromosome 20 contains a region with numerous genes expressed in the epididymis, which are thought important for seminal production, and some viewed as potential targets for male contraception. The PRNP gene encoding the prion protein associated with spongiform encephalopathies, like Creutzfeldt-Jakob disease, is found on chromosome 20. Amyotrophic lateral sclerosis, spinal muscular atrophy, ring chromosome 20 epilepsy syndrome and Alagille syndrome are also associated with chromosome 20. The C20orf96 gene product has been provisionally designated C20orf96 pending further characterization.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is C20orf96 Polyclonal Antibody, Cy3 Conjugated (bs-15120R-Cy3) validated for, and at what dilution?

bs-15120R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does C20orf96 Polyclonal Antibody, Cy3 Conjugated (bs-15120R-Cy3) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse.

How should bs-15120R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise C20orf96 Polyclonal Antibody, Cy3 Conjugated (bs-15120R-Cy3)?

KLH conjugated synthetic peptide derived from human C20orf96

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-15120R-Cy3)?

Cy3 on bs-15120R-Cy3: excitation / emission 512,550nm/570,615nm.