EpCAM Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1513r-bf488-100ul |
| Product Name | EpCAM Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EpCAM |
| Immunogen Range | 221-314/314 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4072 |
| Swiss Prot | P16422 |
| Subcellular location | Cell membrane |
| Synonyms | ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP4; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; hEGP314; KS 1/4 antigen; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD326; EPCAM; GA733-2; M1S2 |
| Background | May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EpCAM Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1513R-BF488) validated for, and at what dilution?
bs-1513R-BF488 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EpCAM Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1513R-BF488) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-1513R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EpCAM Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1513R-BF488)?
KLH conjugated synthetic peptide derived from human EpCAM Immunogen range: 221-314/314.
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-1513R-BF488)?
AbBy Fluor-488 on bs-1513R-BF488: excitation / emission 499nm/519nm.