CD7 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1520R-Cy3-100uL |
| Product Name | CD7 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
FCM
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 924 |
| Swiss Prot | P09564 |
| Subcellular location | Cell membrane |
| Synonyms | GP40; TP41; Tp40; LEU-9; T-cell antigen CD7; T-cell leukemia antigen; T-cell surface antigen Leu-9; CD7 |
| Background | CD7 is a 40 kDa transmembrane, single-chain glycoprotein, which is a member of the immunoglobulin superfamily. CD7 is expressed by the majority of thymocytes and mature T cells, NK cells and pre-B cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. The function of CD7 is not yet known although cross-linking of CD7 with induces a T cell transmembrane calcium flux and CD7 expression is induced by ionomycin. The CD7 molecule has been reported to be the receptor for the IgM-Fc portion (FcR mu) on the surface of T cells. |
| Application Dilution | |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is CD7 Polyclonal Antibody, Cy3 Conjugated (bs-1520R-Cy3) validated for, and at what dilution?
bs-1520R-Cy3 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD7 Polyclonal Antibody, Cy3 Conjugated (bs-1520R-Cy3) react with?
Tested reactivity: Human, Mouse.
How should bs-1520R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD7 Polyclonal Antibody, Cy3 Conjugated (bs-1520R-Cy3)?
KLH conjugated synthetic peptide derived from human CD7
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1520R-Cy3)?
Cy3 on bs-1520R-Cy3: excitation / emission 512,550nm/570,615nm.