phospho-ERK1 + 2 (Thr183/Tyr185) Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1522R-PE |
| Product Name | phospho-ERK1 + 2 (Thr183/Tyr185) Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse ERK2 around the phosphorylation site of Thr183 and Tyr 185 |
| Modification Site | Thr183/Tyr 185 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26413 |
| Swiss Prot | P63085 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ERK; Erk2; MAPK2; PRKM2; Prkm1; C78273; p41mapk; p42mapk; AA47128; AU18647; 93612K14Rik; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; Mapk1; Mapk |
| Background | Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is phospho-ERK1 + 2 (Thr183/Tyr185) Polyclonal Antibody, PE Conjugated (bs-1522R-PE) validated for, and at what dilution?
bs-1522R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-ERK1 + 2 (Thr183/Tyr185) Polyclonal Antibody, PE Conjugated (bs-1522R-PE) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Horse, Chicken, Rabbit, GuineaPig.
How should bs-1522R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-ERK1 + 2 (Thr183/Tyr185) Polyclonal Antibody, PE Conjugated (bs-1522R-PE)?
KLH conjugated synthetic phosphopeptide derived from mouse ERK2 around the phosphorylation site of Thr183 and Tyr 185
What are the excitation and emission wavelengths of the PE conjugate (bs-1522R-PE)?
PE on bs-1522R-PE: excitation / emission 496,564nm/578nm.