GlyR alpha 1/2/3 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1524r-cy5-100uL |
| Product Name | GlyR alpha 1/2/3 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GlyR alpha 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | glycine receptor alpha 1; glycine receptor alpha 2; glycine receptor alpha 3; GLRA1; GLRA2; Glycine receptor 48 kDa subunit; Glycine receptor alpha 2; Glycine receptor subunit alpha 3; Glycine receptor subunit alpha 2; Glycine receptor, alpha 1 subunit; Glycine receptor, alpha 2 subunit; MGC138878; MGC138879; STHE;alpha 1 Glycine Receptor;GLRA1; GLRA1_HUMAN; Glycine receptor 48 kDa subunit; Glycine receptor alpha 1; Glycine receptor strychnine-binding subunit; Glycine receptor subunit alpha-1; Glycine receptor, alpha 1 subunit; HKPX1; STHE; GLR; Glra2; GLRA2_HUMAN; Glycine receptor alpha 2 polypeptide; Glycine receptor alpha 2 subunit; Glycine receptor subunit alpha 2; Glycine receptor subunit alpha-2; Gycine receptor alpha 2. |
| Background | Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation. Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is GlyR alpha 1/2/3 Polyclonal Antibody, Cy5 Conjugated (bs-1524R-Cy5) validated for, and at what dilution?
bs-1524R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GlyR alpha 1/2/3 Polyclonal Antibody, Cy5 Conjugated (bs-1524R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-1524R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GlyR alpha 1/2/3 Polyclonal Antibody, Cy5 Conjugated (bs-1524R-Cy5)?
KLH conjugated synthetic peptide derived from human GlyR alpha 1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-1524R-Cy5)?
Cy5 on bs-1524R-Cy5: excitation / emission 625,650nm/670nm.